Common Location of Determinants in Initiator Transfer RNAs for Initiator-Elongator Discrimination in Bacteria and in Eukaryotes
نویسندگان
چکیده
منابع مشابه
Common location of determinants in initiator transfer RNAs for initiator-elongator discrimination in bacteria and in eukaryotes.
Initiator tRNAs are used exclusively for initiation of protein synthesis and not for elongation. We show that both Escherichia coli and eukaryotic initiator tRNAs have negative determinants, at the same positions, that block their activity in elongation. The primary negative determinant in E. coli initiator tRNA is the C1xA72 mismatch at the end of the acceptor stem. The primary negative determ...
متن کاملInitiator-elongator discrimination in vertebrate tRNAs for protein synthesis.
Initiator tRNAs are used exclusively for initiation of protein synthesis and not for the elongation step. We show, in vivo and in vitro, that the primary sequence feature that prevents the human initiator tRNA from acting in the elongation step is the nature of base pairs 50:64 and 51:63 in the TpsiC stem of the initiator tRNA. Various considerations suggest that this is due to sequence-depende...
متن کاملFrom elongator tRNA to initiator tRNA.
We show that the two most important properties needed for a tRNA to function in initiation in Escherichia coli are its ability to be formylated and its ability to bind to the ribosomal P site. This conclusion is based on conversion of two different elongator tRNAs to ones that can act as initiators in E. coli. We transplanted the features unique to E. coli and eubacterial initiator tRNAs to E. ...
متن کاملLocation alters tRNA identity: Trypanosoma brucei's cytosolic elongator tRNAMet is both the initiator and elongator in mitochondria.
A a rule, protein synthesis is initiated with methionine or formyl methionine from the first AUG codon in an ORF and that AUG is recognized by an initiator tRNAMet-i. Internal AUG codons are then translated by a second tRNA, the elongator tRNAMet-e (1). In eubacteria, mitochondria, and chloroplasts, the tRNAMet-i is charged with methionine, and subsequently the methionine is formylated by a met...
متن کاملpragmatic transfer in compliment responses and chastisements by iranian learners of english
فرهنگ های گوناگون دریافت ها و تعبیر های مختلفی از تناسب و ادب دارند. بنابراین، ارتباط میان فرهنگی خطرات ذاتی خطای ارتباطی را نشان می دهد. این پژوهش به منظور تعیین وقوع انتقال منظورشناختی زبان در زبان بینابینی زبان آموزان ایرانی، اختلاف منظورشناختی زبان را بین فارسی و انگلیسی در کنش های کلامی پاسخ تحسین و انتقاد بررسی می کند.داده ها از میان 33 آمریکایی انگلیسی زبان، 85 زبان آموز ایرانی در سطوح م...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m212890200